Principles of Plant Biotechnology ( AG206 )

Principles of Plant Biotechnology ( AG206 )

Concepts of Plant Biotechnology: History of Plant Tissue Culture and Plant Genetic Engineering; Scope and importance in Crop Improvement: Totipotency and Morphogenesis, Nutritional requirements of in-vitro cultures; Techniques of in-vitro cultures, Micro propagation, Anther culture, Pollen culture, Ovule culture, Embryo culture, Test tube fertilization, Endosperm culture, Factors affecting above in-vitro culture; Applications and Achievements; Somaclonal variation, Types, Reasons: Somatic embryogenesis and synthetic seed production technology; Protoplast isolation, Culture, Manipulation and Fusion; Products of somatic hybrids and cybrids, Applications in crop improvement. Genetic engineering; Restriction enzymes; Vectors for gene transfer – Gene cloning – Direct and indirect method of gene transfer – Transgenic plants and their applications. Blotting techniques – DNA finger printing – DNA based markers – RFLP, AFLP, RAPD, SSR and DNA Probes – Mapping QTL – Future prospects. MAS, and its application in crop improvement.

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